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Figure 1: Bovine fetal spleen cell culture (Enlarged view). FIG. 1. BFS: unstained bovine fetal spleen. This continuous cell culture, now in the 40th passage, was observed with the 20X objective (200X total magnification) using standard optical microscopy. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 2: HeLa cell culture (Enlarged view). FIG. 2. HeLa: the first aneuploid epithelial-like cell culture to be derived from human tissue and maintained continuously by serial cell culture. This cell line was isolated from an adenocarcinoma of the cervix of a 31-year-old female in 1951. Unstained cells were observed with the 20X objective (200X total magnification) using standard optical microscopy. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 3: Rhesus monkey kidney cell culture (Enlarged view). FIG. 3. MA104: unstained fetal Rhesus monkey kidney cell culture. This continuous cell culture was observed with the 20X objective (200X total magnification) using standard optical microscopy. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 4: Madin-Darby bovine kidney cell culture (Enlarged view). FIG. 4. MDBK: unstained Madin-Darby bovine kidney. This continuous cell culture was derived from the kidney of an apparently normal adult steer in 1957 and was observed with the 20X objective (200X total magnification) using standard optical microscopy. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 5: Mouse L cell culture (Enlarged view). FIG. 5. Mouse L: a continuous cell culture derived from mouse embryo fibroblasts that were treated with methyl cholanthrene 3, a carcinogenic agent.  Unstained cells were observed with the 20X objective (200X total magnification) using standard optical microscopy. (Lori Phalen and Carol Blair, Colorado State University, Fort Collins, CO)

Figure 6: Cell culture from Aedes albopictus (Enlarged view). FIG. 6. C6:36: a continuous cell culture derived from Aedes albopictus mosquito larvae. These unstained cells were observed with the 20X objective (200X total magnification) using standard optical microscopy. (Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 7: Bovine fetal spleen cell culture (Enlarged view). FIG. 7. BFS: May-Grunwald Giemsa-stained bovine fetal spleen cells. This uninfected primary cell culture was observed with a 10X objective (100X total magnification) using standard optical microscopy.  For a description of the May-Grunwald Giemsa stain please see the CPE protocol. (Carol Blair and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 8: Bovine nasal turbinate cell culture (Enlarged view). FIG. 8. BNT: Giemsa-stained bovine nasal turbinate cells. The uninfected primary cell culture was observed with a 10X objective (100X total magnification) using standard optical microscopy. For a description of the Giemsa stain please see the CPE protocol. (Carol Blair and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 9: Vero cell culture (Enlarged view). FIG. 9. Vero: Giemsa-stained Vero cells (from an African green monkey kidney). This continuous cell culture was observed with a 10X objective (100X total magnification) using standard optical microscopy. (Carol Blair and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 10: HeLa cell culture (Enlarged view). FIG. 10. HeLa: Giemsa-stained HeLa cells (from a human cervical carcinoma). This continuous uninfected cell culture was observed with a 10X objective (100X total magnification) using standard optical microscopy.  Note: uninfected HeLa cells demonstrate a lack of cell contact inhibition that is easily mistaken for CPE. (Carol Blair and Erica Suchman, Colorado State University, Fort Collins, CO)

Figure 11: Human intestinal adenocarcinoma cell culture (Enlarged view). FIG. 11. HCT-8: a human intestinal adenocarcinoma continuous cell culture. These unstained cells were observed with the 20X objective (200X total magnification) using standard optical microscopy. (Mary Morada,  Pace University, New York, NY)

Figure 12: Human neuroblastoma cell culture (Enlarged view). FIG. 12. SK-N-SH: human neuroblastoma cells. These unstained cells were observed using phase-contrast microscopy at  128X magnification. (Emmanuel Brako, Winona State University, Winona, MN and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)

Figure 13: Rabbit kidney cell culture (Enlarged view). FIG. 13. LLC-RK1: rabbit kidney cells. These unstained cells were observed using phase-contrast microscopy at 320X magnification. (Emmanuel Brako, Winona State University, Winona, MN and Linda Phaire-Washington, Argonne National Laboratory, Chicago, IL)